The Rosa26 locus on mouse chromosome 6 is often used to integrate transgenic structures to achieve ubiquitous or conditional gene expression in mice and is considered to be one of the ideal locations for knockin targeted genes. The DNA fragments placed at the Rosa26 site are very safe and stable. Using the classical gene targeting method, obtain a gene knockin mouse at Rosa26 always takes one year or more. With the application of CRISPR/Cas9-mediated knockin technology, large DNA fragments can be accurately and efficiently inserted into the Rosa26.
https://www.creative-biogene.c....om/Services/Stable-c

CRISPR/Cas9 PlatformCB, a professional gene-editing technology services company, has extensive experience in the application of CRISPR/Cas gene-editing technology and has successfully knocked out/knocked specific genes in >300 mice. We guarantee to provide you with at least 2 Founder mice or 3 F1 mice and complete them within 3-5 months.

Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing. It has been widely used in current biology, functional genome screening, cell-based human hereditary disease modeling, epigenomic studies and visualization of cellular processes.

CRISPR/Cas9 system consists of a “guide” RNA (gRNA) and a bacterial CRISPR-associated endonuclease (Cas9). The gRNA is a short synthetic RNA composed of a Cas9-binding “scaffold” sequence and ∼20 nucleotide “targeting” sequence that defines the target genomic site to be modified. Cas9 contains two nuclease domains to induce site-specific DNA cleavage. It’s a scalable genome-wide editing technology for its ease of generating gRNAs. The simplicity and high-efficiency of CRISPR/Cas9 system make it a preferable genomic knockout method to the traditional ZFN and TALEN system. Our scientists are experts at performing gene knockout with CRISPR/Cas9, from designing gRNA constructs to transfection and single clone generation of a wide range of cells, including difficult-to-transfect and tumor cell lines.
https://www.creative-biogene.c....om/Services/Plasmid-